Training Course
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Training Course
EPR and Cytogenetic Biodosimetry Training Program
Trainings will be 1-full day on September 23rd, 2024.
EPR Training Course
- Venue: The 50th Memorial Auditorium, Hirosaki Uni.
- Address: 1 Bunkyo-cho, Hirosaki-shi, Aomori-ken, 036-8560 Japan
- Date: Monday, September 23rd, 2024, from 8:00 to 18:00
- Participation fee: 10,000 JPY
- On-site in vivo EPR tooth dosimetry system: Morning tutorial and afternoon practical session
- Comprehensive EPR courses, including an overview of advanced techniques and hands-on training in Easyspin techniques; onsite supply of in vivo tooth EPR instrument
Objectives
- Morning didactic lectures present a basic overview of the principles of EPR spectroscopy and instrumentation; provide an introduction to advances in EPR instrumentation, spectral acquisition, and analysis
- Afternoon parallel EPR practical sessions offer trainees the opportunity for hands-on familairization and operation of in vivo tooth dosimeter and learning the basics of quantitative EPR methods
Time course
| From | To | Contents | |
| 8:00 | 9:00 | Introduction, history of EPR spectroscopy, History and basic concepts of EPR dosimetry of teeth, nails and bone, in vitro and in vivo (Hal Swartz) | |
| 9:00 | 10:00 | The EPR spectrometer (Bridge, cavity) (Jason Sidabras) | |
| 10:00 | 10:30 | Break | |
| 10:30 | 11:15 | Advanced EPR technologies relevant to dosimetry (Rapid scan, multi-harmonic detection...) (Jason Sidabras) | |
| 11:15 | 12:45 | Introduction to Easyspin (Maurizio Marrale) | |
| 12:45 | 14:00 | Lunch | |
| Afternoon: Parallel Practical Sessions 1-2, trainees divided into Groups 1 and 2 | |||
| Group 1 | Group 2 | ||
| 14:00 | 15:30 | In vivo tooth EPR dosimetry (Hal Swartz, Ichiro Yamaguchi, Jason Sidabras) | Benchtop EPR spectrometer and basics in EPR quantification (François Trompier and Maurizio Marrale) |
| 15:45 | 17:15 | Benchtop EPR spectrometer and basics in EPR quantification (François Trompier and Maurizio Marrale) | In vivo tooth EPR dosimetry (Hal Swartz, Ichiro Yamaguchi, Jason Sidabras) |
| 17:15 | 18:00 | Exchange with expert panel, question/answer period, feedback | |
Cytogenetic Training Course
- Venue: Graduate School of Health Sciences, Hirosaki Uni.
- Address: 66-1 Hon-cho, Hirosaki-shi, Aomori-ken, 036-8564, Japan
- Date: Monday, September 23rd, 2024, from 9:00 to 17:30
- Participation fee: 10,000 JPY
- Chemical-induced premature chromosome condensation (PCC) assay
- FISH translocation assay using #1-2-4 WCP probe
- Technical update by IREM, Hirosaki University
Objectives
Proficiency in performing the fundamental procedures of the PCC-ring assay, utilized in dosimetry for patients subjected to high-dose exposure, and expertise in using the FISH-translocation assay for retrospective dosimetry.
Prior to training
Hirosaki University will prepare the following:
- Cultured blood (10 Gy-X-ray) for chemical-induced PCC assay training
- Cells for FISH-Tr assay training
- Probe-mounted chromosomes for FISH-Tr assay training
- Fixed cells for sharing modified techniques (enriching metaphase cells)
- Improved C-banding (cTAG) slides
Time course
| From | To | Contents |
| 9:00 | 9:15 | Lab installation and orientation |
| 9:15 | 10:00 | WCP-probe mounting and incubation at 37°C |
| 10:00 | 10:10 | Addition of calyculin A for PCC assay |
| 10:10 | 10:30 | Short break |
| 10:30 | 12:00 | Harvest of cultured blood for PCC assay |
| 12:00 | 13:00 | Lunch |
| 13:00 | 14:00 | FISH continued (washing and counterstain with DAPI) |
| 14:00 | 14:30 | Technical update: enriching metaphase cells |
| 14:30 | 15:00 | Giemsa staining and drying |
| 15:00 | 15:20 | Short break |
| 15:20 | 15:45 | Image capturing by Metafer |
| 15:45 | 17:00 | Microscope and image observation
|
| 17:00 | 17:25 | Discussion |
| 17:25 | 17:30 | Closing remarks |